皖西白鹅育肥期肌肉脂肪酸组成及肝PPARα、FADS2和ME1基因表达规律的研究

doi:10.11843/j.issn.0366-6964.2017.10.014

Abstract

Abstract:

This study aimed to elucidate the fatty acids content in muscles and fatty acid synthesis related gene expression profile in muscle of Wanxi White geese during fattening period. A total of 60 geese were selected at 70 d of age with similar body weight. The geese were divided into 3 replicates with 20 per replicate. Five geese were randomly selected from each replicate for breast muscle, leg muscle and liver sampling with 10 d interval during fattening till 100 d of age. Gas chromatography was used for fatty acid content determination and RT-PCR was used for PPARα, FADS2 and ME1 gene expression levels determination. The results showed that:1) Saturated fatty acid content in breast muscle was higher than that in leg muscle (P<0.000 1). Monounsaturated fatty acids and polyunsaturated fatty acids were lower in breast muscle than that in leg muscle (P=0.044, P=0.017). The content of C16:1 and C18:2 were increased, C18:0 was decreased during fattening. 2) The expression of PPARα was the highest on 20 d and the lowest on 30 d (P<0.000 1) in fattening period. The expression of FADS2 was the highest on 10 d (P=0.009) in fattening period. The expression of ME1 was lowest on 30 d (P=0.010) in fattening period. 3) The expression of PPARα increasing was associated with the increasing C16:1, C18:3 and UFA/SFA contents while the content of SFA decreased. The expression of FADS2 increasing was associated with the increasing C16:1, C18:1, C18:3, MUFA and UFA/SFA contents, while SFA and PUFA decreased. The expression of ME1 increasing was associated with the increasing C16:1, C18:1, C18:3, MUFA and UFA/SFA contents, while C18:0, C20:3 and SFA contents decreased. The expression levels of PPARα, FADS2 and ME1 were significantly associated with fatty acids content in Chinese Wanxi White geese, suggesting that these genes might be used as candidate genes for fatty acid selection in geese breeding.

CLC Number:

S835.4

CHEN Xing-yong, ZHAO Ning, ZHANG Yan, GENG Zhao-yu. The Fatty Acid Profile in Muscles and Expression of PPARα, FADS2 and ME1 Genes in Liver of Chinese Wanxi White Geese in Fattening Period[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1912-1919.

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The Fatty Acid Profile in Muscles and Expression of PPARα, FADS2 and ME1 Genes in Liver of Chinese Wanxi White Geese in Fattening Period

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